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SNAP-Surface® 488

SNAP-Surface® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells.

  • This is a cell impermeable substrate (BG-488) based on ATTO-TEC dye ATTO 488
  • It is suitable for standard fluorescein filter sets
  • It has an excitation maximum at 506 nm and emission maximum at 526 nm

Ordering Information

$name
  • 50 nmol
    $307.00
  • Product Information
    This package includes 50 nmol of SNAP-Surface 488, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1: Live HEK293 cells transiently transfected with pSNAP-ADRβ2

    Figure 1: Live HEK293 cells transiently transfected with pSNAP-ADRβ2
    Cells were labeled with SNAP-Surface 488 (green) for 30 minutes.

    Figure 2: Structure of SNAP-Surface 488 (MW 841.9 g/mol)

    Figure 2: Structure of SNAP-Surface 488 (MW 841.9 g/mol)

    Figure 3: Excitation (dotted line) and emission (solid line) spectra of SNAP-Surface 488 coupled to SNAP-tag in buffer at pH 7.5 Figure 3: Excitation (dotted line) and emission (solid line) spectra of SNAP-Surface 488 coupled to SNAP-tag in buffer at pH 7.5
    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Surface,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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