SNAP-Surface® 549 is a photostable red fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells.
- It is a cell impermeable substrate (BG-549) based on the Dyomics dye DY-549P1
- It is suitable for use with standard TAMRA or Cy3 filter sets
- It has an excitation maximum at 560 nm and emission maximum at 575 nm
This package includes 50 nmol of SNAP-Surface 549, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.
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