SNAP-Surface® 549

SNAP-Surface® 549 is a photostable red fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells.

  • It is a cell impermeable substrate (BG-549) based on the Dyomics dye DY-549P1
  • It is suitable for use with standard TAMRA or Cy3 filter sets
  • It has an excitation maximum at 560 nm and emission maximum at 575 nm

Ordering Information

$name
  • 50 nmol
    $307.00
  • Product Information
    This package includes 50 nmol of SNAP-Surface 549, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1: Structure of SNAP-Surface 549 (MW 1195.2 g/mol)


     
    Figure 2: Excitation (dotted line) and emission spectra of SNAP-Surface 549 in buffer at pH 7.5 Figure 2: Excitation (dotted line) and emission spectra of SNAP-Surface 549 in buffer at pH 7.5
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    Figure 3: Structure of SNAP-Surface 549 (MW 1195.2 g/mol)
    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Surface,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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