SNAP-Biotin® is suitable for applications such as biotinylation of SNAP-tag® fusion proteins in living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western blot or for capture with streptavidin for binding and interaction studies.
- It is a cell-permeable substrate (BG-Biotin)
- It is based on biotin with an amidocaproyl linker
This package contains 50 nmol of SNAP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of SNAP-tag fusion proteins in cells.
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.
There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-Biotin substrate is described below.
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.