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SNAP-Biotin®

SNAP-Biotin® is suitable for applications such as biotinylation of SNAP-tag® fusion proteins in living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western blot or for capture with streptavidin for binding and interaction studies.

  • It is a cell-permeable substrate (BG-Biotin)
  • It is based on biotin with an amidocaproyl linker
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Catalog # Concentration Size List Price Your Price Quantity
S9110S Not Applicable 50 nmol $288.00
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  • Product Information
    This package contains 50 nmol of SNAP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of SNAP-tag fusion proteins in cells.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-Biotin substrate is described below.
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    Figure 1: Structure of SNAP-Biotin (MW 609.7 g/mol)
    This product is related to the following categories:
    Biotin Labels Products,
    SNAP-tag Substrates Products ,
    Protein Labeling Products
    This product can be used in the following applications:
    SNAP Cell,
    In vivo Imaging,
    Pulse Chase,
    Receptor Internalization,
    Protein Localization,
    Protein Labeling Snap Clip
    • Reagents Supplied
    • Properties & Usage
    • Product Notes
  • Protocols, Manuals & Usage
  • Tools & Resources
  • FAQs & Troubleshooting
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    • Certificate of Analysis
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