SNAP-Cell® Block

SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells.

  • SNAP-Cell Block is highly membrane permeable
  • Once it reacts in the cell with the SNAP-tag, it irreversibly inactivates it for subsequent labeling steps
  • It can be used to generate inactive controls in live cell labeling experiments performed with SNAP-tag fusion proteins

Ordering Information

$name
  • 100 nmol
    $121.00
  • Product Information
    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the instructions supplied with SNAP-tag plasmids. The labeling of SNAP-tag fusion proteins with SNAP-Cell substrates is described in the instructions supplied with SNAP-Cell substrates. The use of SNAP-Cell Block during the labeling of fusion proteins with SNAP-Cell substrates is described below.

    Figure 1: Structure of SNAP-Cell Block (MW 338.2 g/mol)
    figure 1: Structure of SNAP-Cell Block (MW 338.2 g/mol)


    Product Categories:
    Blocking Agents Products,
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Cell,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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