SNAP-Cell® Oregon Green®
SNAP-Cell® Oregon Green® is a photostable green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells. Conjugates of Oregon Green are more photostable than those of fluorescein, and their fluorescence properties are essentially pH insensitive in the physiological pH range.
- It is a cell-permeable substrate (BG-Oregon Green) based on the Invitrogen dye, Oregon Green
- It is suitable for standard fluorescein filter sets
- It has an excitation maximum at 490 nm and an emission maximum at 514 nm
This package contains 50 nmol of SNAP-Cell Oregon Green substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.
The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the dye-substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.
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