SNAP-Cell® Oregon Green®

SNAP-Cell® Oregon Green® is a photostable green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells. Conjugates of Oregon Green are more photostable than those of fluorescein, and their fluorescence properties are essentially pH insensitive in the physiological pH range.

  • It is a cell-permeable substrate (BG-Oregon Green) based on the Invitrogen dye, Oregon Green
  • It is suitable for standard fluorescein filter sets
  • It has an excitation maximum at 490 nm and an emission maximum at 514 nm

Ordering Information

$name
  • 50 nmol
    $339.00
  • Product Information
    This package contains 50 nmol of SNAP-Cell Oregon Green substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the dye-substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag. 
     
    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below. 

    Figure 1: Live U2OS cells transiently transfected with pSNAP-Cox8A (mitochondrial expression).

    Figure 1: Live U2OS cells transiently transfected with pSNAP-Cox8A (mitochondrial expression).
    Cells were labeled with SNAP-Cell Oregon Green (green) for 15 minutes and counterstained with Hoechst 33342 (blue).
    Figure 2: 
    Excitation (dotted line) and emission spectra after coupling of SNAP-Cell Oregon Green to SNAP-tag in buffer at pH 7.5.
    Figure 2:  Excitation (dotted line) and emission spectra after coupling of SNAP-Cell Oregon Green to SNAP-tag in buffer at pH 7.5.

    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    In vivo Imaging,
    Protein Localization,
    Protein Labeling (SNAP/CLIP)
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