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  • m7G(5')ppp(5')G RNA Cap Structure Analog

    Description

    The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

    A method for efficient in vitro synthesis of capped RNA using E.coli RNA polymerase primed with m7G(5´ )ppp(5´ )G (#S1404S) or m7G(5´ )ppp(5´ )A (#S1405S) has been developed by Contreas et al. (7).

    Sequence

    7mG(5´)ppp(5´)G

    Properties and Usage

    Storage Temperature

    -20°C

    Supplied As

    Lyophilized Sodium Salt

    Suggested Working Concentration

    10mM

    Recommended Resuspension Volume

    100 μl

    Notes

    1. Addition of 100 µl of water gives approximately a 10 mM solution.

    References

    1. Shatkin, A.J. (1978). Cell. 9, 645-653.
    2. Fillipowicz, W. (1978). FEBS Lett.. 96, 1-11.
    3. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
    4. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
    5. Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
    6. Konarska, M.M. et al. (1984). Cell. 38, 731-736.
    7. Contreas, R. et al. (1982). Nucl. Acids Res.. 10, 6353-2363.
    8. Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.

    Supporting Documents

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