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m7G(5')ppp(5')G RNA Cap Structure Analog

Ordering Information

  • 1 μmol
    $149.00
  • 5 μmol
    $596.00
  • Product Information
    The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

    A method for efficient in vitro synthesis of capped RNA using E.coli RNA polymerase primed with m7G(5´ )ppp(5´ )G (#S1404S) or m7G(5´ )ppp(5´ )A (#S1405S) has been developed by Contreas et al. (7).

    Sequence

    7mG(5´)ppp(5´)G
    Product Categories:
    RNA Synthesis Products
    Applications:
    In vitro Synthesis & Processing,
    RNA Cloning,
    RNA Characterization & Modification
    • Properties and Usage
    • Notes
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