SbfI
This product has been replaced with the high fidelity version, SbfI-HF™ (NEB #R3642).
- Replaced by SbfI-HF® on December 15, 2021
- Catalog # R0642 was discontinued on December 15, 2021
- Time-Saver™ qualified for digestion in 5-15 minutes
- 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
- High Fidelity (HF®) version available (NEB #R3642) supplied with rCutSmart Buffer
- Restriction Enzyme Cut Site: CCTGCA/GG
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Product Information
SbfI has an High Fidelity version, SbfI-HF® (NEB #R3642)
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the SbfI gene from Streptomyces species Bf-61 (S.K. Degtyarev).- This product is related to the following categories:
- Discontinued (<3 years)
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Product Citation Tool
Additional Citations
- Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving, polymorphic and fuzzy boundary of a plant pseudoautosomal region. Mol Ecol; Jan;25(1), 414-30.
- Qiu S, Bergero R, Guirao-Rico S, Campos JL, Cezard T, Gharbi K, Charlesworth D. (2016) RAD mapping reveals an evolving polymorphic and fuzzy boundary of a plant pseudoautosomal region Mol Ecol; Jan;25(1), 414-30.
- Díaz-Arce N, Arrizabalaga H, Murua H, Irigoien X, Rodríguez-Ezpeleta N. (2016) RAD-seq derived genome-wide nuclear markers resolve the phylogeny of tunas. Mol Phylogenet Evol; Sep;102, 202-7.
- Araneda C, Larraín MA, Hecht B, Narum S. (2016) Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments. Ecol Evol; Apr 26,
- Rutledge LY, Devillard S, Boone JQ, Hohenlohe PA, White BN. (2015) RAD sequencing and genomic simulations resolve hybrid origins within North American Canis. Bio Lett; Jul;11(7)., pii: 20150303. .
- Liu S, Vallejo RL, Palti Y, Gao G, Marancik DP, Hernandez AG, Wiens GD. (2015) Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout. Front Genet; Sep 24;6:298.
- Mathers TC, Hammond RL, Jenner RA, Hänfling B, Atkins J, Gómez A. (2015) Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity (Edinb); Jul;115(1), 37-46.
- Zhou Z, Liu S, Dong Y, Gao S, Chen Z, Jiang J, Yang A, Sun H, Guan X, Jiang B, Wang B. (2015) High-Density Genetic Mapping with Interspecific Hybrids of Two Sea Urchins, Strongylocentrotus nudus and S. intermedius, by RAD Sequencing. PLoS One; Sep 23;10(9),
- Longo G, Bernardi G. (2015) The evolutionary history of the embiotocid surfperch radiation based on genome-wide RAD sequence data. Mol Phylogenet Evol; Jul;88, 55-63.
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Quality, Safety & Legal
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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Double Digestion with NEBcloner
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