α-Lytic Protease
cloned at NEB 37 95 Heat

P8113 Cutsite

α-Lytic Protease (aLP) cleaves after Threonine (T), Alanine (A), Serine (S) and Valine (V) residues. Its specificity makes it an orthogonal and alternative protease to others commonly used in proteomics applications, including trypsin and chymotrypsin.

  • Analyze complex proteomes
  • Suitable for both in-gel and solution digests
  • Optimal activity and stability for up to 24 months
  • Ideal for digestion of proteins for proteomic analysis by mass spectrometry
Catalog # Concentration Size List Price Your Price Quantity
P8113S 0.4 mg/ml 20 µg $89.00
P8113L 0.4 mg/ml 100 µg $339.00
Please enter a quantity for at least one size
  • Product Information

    α-Lytic Protease (aLP) cleaves after Threonine (T), Alanine (A), Serine (S) and Valine (V) residues. Its specificity makes it an orthogonal and alternative protease to others commonly used in proteomics applications, including trypsin and chymotrypsin. Peptides generated by aLP are of similar average length to those of Trypsin.

    Figure 1.  α-Lytic Protease can be used alone or in combination with other proteases to yield increased sequence coverage.



    Comparison of sequence coverage for three protein standards after parallel digestion using Trypsin (blue) and a-Lytic Protease (gold). The combined data set (grey) results in overlapping peptides and increased sequence coverage.

    Product Source

    Purified from Lysobacter enzymogenes

    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    Product Categories:
    Proteases Products
    Applications:
    Protein Analysis Tools,
    Protein Digestion,
    Proteomics
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