Endoproteinase GluC

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P8100S     -20       Endoproteinase GluC P8100SVIAL -20 1 x 50 µg Not Applicable   GluC Reaction Buffer B8100SVIAL -20 1 x 1.5 ml 2 X

Companion Products

• Trypsin-ultra™, Mass Spectrometry Grade
• Endoproteinase AspN
• Endoproteinase LysC
• α-Lytic Protease

1. Storage conditions: Supplied in dry format from Tris-HCl and sodium chloride buffer.
2. Repeated freeze/thawing is not recommended.
3. GluC may also be used in 20-50mM phosphate or ammonium bicarbonate buffer. For optimal activity, supplement reactions with Glu-Glu dipeptide. Glutamic acid residues are highly favored in Tris, phosphate and ammonium bicarbonate buffers.
4. GluC is purified with a His-tag at its C-terminal of the protein. The predominant species has four histidine residues at the C-terminus with the remaining species showing a distribution from one to six His tags.

1. Drapeau, G.R., Boily, Y. and Houmard, J. (1972). Purification and properties of an extracellular protease of Staphylococcus aureus. J. Biol. Chem.. 247, 6720-26.
2. Benner, J.S., Martin, D. and Schampel, A.K. unpublished results.
3. Birktoft, J.J. and Breddam, K. (1994). Proteolytic Enzymes: Serine and Cysteine Peptidases. A.J. Barrett(Ed.), Glutamyl endopeptidases. Methods Enzymol.. 244, 114-126. San Diego: Academic Press.
4. Breddam, K. and Meldal, M. (1992). Substrate preference of glutamic-acid-specific endopeptidases assessed by synthetic peptide substrates based on intramolecular fluorescence quenching. Eur. J. Biochem.. 206, 103-7.

1. Typical GluC Digest Reaction Conditions (P8100)
2. Typical Trypsin and GluC Co-Digest Reaction Conditions (P8100)
3. Typical GluC In-Gel Digest Reaction Conditions (P8100)

• Protease Selection Chart

1. What is the stability/compatibility of Endoproteinase GluC with regards to presence of DTT, urea, concentrations of methanol, acetonitrile, nitrosoguanidine, thiourea and glycerol?
2. How does one carry out limited proteolysis experiments with Trypsin and Endoproteinase Glu-C? Which chemicals can be used to quench the reactions at given time points to monitor proteolysis progress via SDS- PAGE?

• Glycoproteomics Technical Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P8100S_v1
• P8100S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P8100S_v1_0051706
• P8100S_v1_0051712
• P8100S_v1_10018423
• P8100S_v1_10028354
• P8100S_v1_10041129
• P8100S_v1_10042496
• P8100S_v1_10043441
• P8100S_v1_10057979
• P8100S_v1_10065472
• P8100S_v1_10079350
• P8100S_v1_10085155
• P8100S_v1_10089233
• P8100S_v1_10101725
• P8100S_v1_10115669
• P8100S_v2_10128983
• P8100S_v2_10140279
• P8100S_v2_10147026
• P8100S_v2_10153428
• P8100S_v2_10164822
• P8100S_v2_10179172
• P8100S_v2_10195255
• P8100S_v2_10216778
• P8100S_v2_10231455
• P8100S_v2_10236804
• P8100S_v2_10245220
• P8100S_v2_10259266
• P8100S_v2_10270381

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Endoproteinase GluC
• GluC Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.