Factor Xa Protease

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Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.

  • Compatible for use with the pMAL™ Protein Fusion and Purification System (NEB #E8200)
  • Allows for convenient removal of MBP by loading the MBP-fusion digest onto amylose resin and collecting your protein of interest in the flow through

Ordering Information

P8010S_pdp_800X450
  • 1 mg/ml
    50 μg
    $68.00
  • 1 mg/ml
    250 μg
    $272.00
  • Product Information
    Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate (1,2,3). The most common secondary site, among those that have been sequenced, is Gly-Arg. There seems to be a correlation between proteins that are unstable in E.coli and those that are cleaved by Factor Xa at secondary sites; this may indicate that these proteins are in a partially unfolded state (Walker, I., Riggs, P., unpublished observations). Factor Xa will not cleave a site followed by proline or arginine.

    Product Source

    Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell's viper venom.
    Product Categories:
    Proteases
    Applications:
    Protein Digestion
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