RNase B

Description


 
RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.

Product Source

Bovine pancreas

Properties and Usage

Storage Temperature

-20°C

Storage Conditions

50 μl Milli-Q water
pH 7.0 @ 25°C

Molecular Weight

Theoretical: 17000 daltons

References

  1. Plummer, T.H. and Hirs, C. (1964). J. Biol. Chem. 239, 2530-2538.
  2. Plummer, T.H. Jr., Tarentino, A. and Maley, F. (1968). J. Biochem. (Tokyo). 243, 5158-5164.
  3. Liang, C.-J. Yamashita, K. and Kobata, A. (1980). J. Biochem (Tokyo). 88, 51-58.

FAQs

  1. Do detergents inhibit exoglycosidases/endoglycosidases?

Tech Tips

This is a good positive control for Endo H, Endo Hf, PNGase F and PNGase F Glycerol Free
250 µg is enough for approximately 20 reactions

Protocols

  1. RNase B Deglycosylation Protocol (P7817)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.