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  • RNase B

    Description

    RNase B is a high mannose glycoprotein (1-3) that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. RNase B has a single N-linked glycosylation site which makes it ideal for SDS-PAGE gel shift assays.

    Product Source

    Bovine pancreas

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    50 μl Milli-Q water
    pH 7.0 @ 25°C

    Molecular Weight

    Theoretical: 17000 daltons

    References

    1. Plummer, T.H. and Hirs, C. (1964). J. Biol. Chem. 239, 2530-2538.
    2. Plummer, T.H. Jr., Tarentino, A. and Maley, F. (1968). J. Biochem. (Tokyo). 243, 5158-5164.
    3. Liang, C.-J. Yamashita, K. and Kobata, A. (1980). J. Biochem (Tokyo). 88, 51-58.

    FAQs

    1. Do detergents inhibit exoglycosidases/endoglycosidases?

    Tech Tips

    This is a good positive control for Endo H, Endo Hf, PNGase F and PNGase F Glycerol Free
    250 µg is enough for approximately 20 reactions

    Protocols

    1. RNase B Deglycosylation Protocol (P7817)

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.