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  • E. coli Ribosome

    Description

    The ribosome is the machinery for protein synthesis in a cell. The 70S ribosome consists of a small subunit (30S) and a large subunit (50S). This preparation of ribosome is highly active in the PURExpress Protein Synthesis Kit (NEB #E6800).

    Product Source

    The ribosome is purified from an E. coli B strain

    Properties and Usage

    Storage Temperature

    -80°C

    Heat Inactivation

    No

    Notes

    1. Avoid multiple freeze/thaw cycles. If you plan to use the ribosome multiple times, we recommend preparing aliquots after first thaw. Handle the ribosome gently, mix by tapping the tube, and avoid vigorous vortexing or high speed centrifugation. Mg2+ is necessary to keep the ribosome intact. If you are going to dilute the ribosome, be sure to include 10 mM magnesium acetate in the buffer.
    2. In vitro translation
    3. Ribosome structural and functional studies
    4. Target for drug screening
    5. Starting material for isolation of native ribosomal RNAs (5S, 16S and 23S)

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is the storage buffer for the E. coli ribosomes?
    2. Are the ribosomes the same as what is provided in the PURExpress Protein Synthesis kits?
    3. I want to work with the 50S or 30S ribosomal subunits. Are the 50S and 30S ribosomal subunits available dissociated/separately?
    4. How many PURExpress reactions in one pack of E. coli ribosome P0763S?
    5. Detailed FAQs for PURExpress?
    Do not vortex to mix, mix gently
    Avoid freeze/thaw cycles