Bacteroides Heparinase II

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0736S     -80       Bacteroides Heparinase II P0736SVIAL -80 1 x 0.02 ml 4,000 units/ml   Bacteroides Heparinase Reaction Buffer B0735SVIAL -20 1 x 1 ml 10 X
P0736L     -80       Bacteroides Heparinase II P0736LVIAL -80 1 x 0.05 ml 4,000 units/ml   Bacteroides Heparinase Reaction Buffer B0735SVIAL -20 1 x 1 ml 10 X

Companion Products

• Bacteroides Heparinase I
• Bacteroides Heparinase III

1. Avoid repeated freeze-thaw cycles.
2. Reaction Conditions:
   
   1. Combine 10 μl of 1 mg/ml heparin substrate, 10 μl Bacteroides Heparinase Reaction Buffer and H₂O in a total reaction volume of 100 μl. 
   2. Add 1 μl Bacteroides Heparinase II.
   3.  Incubate reaction at 30°C for 1–24 hours (monitor absorbance at 232 nm for determination of partial or complete digestion). 
   Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.

1. What are Bacteroides Heparinase Enzymes?
2. What is the difference between Bacteroides Heparinase I, II and III?
3. Can Bacteroides Heparinase I, II, and III be used together in one digest?
4. What is the optimal pH range for Bacteroides Heparinase II?
5. Is Bacteroides Heparinase II activated by Calcium?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0736S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0736S_L_v1_0031602
• P0736S_L_v1_0031605
• P0736S_L_v1_0031610
• P0736S_L_v1_0031707
• P0736S_L_v1_0031710
• P0736S_L_v1_0031801
• P0736S_L_v1_0031803
• P0736L_v1_10020493
• P0736S_v1_10030741
• P0736L_v1_10039989
• P0736S_v1_10039991
• P0736L_v1_10048140
• P0736S_v1_10048142
• P0736S_v1_10055716
• P0736L_v1_10052582
• P0736L_v1_10062642
• P0736L_v1_10067097
• P0736S_v1_10062644
• P0736L_v1_10070311
• P0736L_v1_10078914
• P0736S_v1_10078916
• P0736L_v1_10081147
• P0736L_v1_10090691
• P0736S_v1_10090694
• P0736S_v1_10102125
• P0736L_v1_10099213
• P0736S_v1_10109984
• P0736L_v1_10109986
• P0736S_v1_10133526
• P0736S_v1_10140327
• P0736L_v1_10145036
• P0736L_v1_10153967
• P0736S_v1_10153970
• P0736L_v1_10181623
• P0736S_v1_10181622
• P0736S_v1_10190589

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Bacteroides Heparinase II
• Bacteroides Heparinase Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.