Bacteroides Heparinase I
Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
- Recombinant enzyme with no detectable glycosidase, sulfatase or uronidase contaminating activities
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 12 months when stored in solution at -80°C
- Optimal for use in coagulation studies
Bacteroides Heparinase I cloned from Bacteroides eggerthii, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
In contrast to the Flavobacterium heparinum Heparinase I which cleaves the glycosidic bond between N-sulfated hexosamines and 2-O-sulfated iduronic acid residues, the Bacteroides Heparinase I cleaves between these same residues as well as the 2-O-sulfated glucuronic acid residues. The 2-O-sulfated uronic acid residue is essential for the activity of Bacteroides Heparinase I and 6-O-sulfation of GlcNS does not hinder enzyme activity. While Bacteroides Heparinase I cleaves 2-O sulfated iduronic acid and 2-O sulfated glucuronic acid residues at similar rates, the Flavobacterium heparinum Heparinase I has a much higher rate of cleavage for 2-O sulfated iduronic acid residues (1). Limited digest of porcine mucosal heparin with Flavobacterium heparinum Heparinase I results in sulfated heparin oligosaccharides structures previously reported (2). Limited digest of porcine mucosal heparin with the Bacteroides Heparinase I results in heparin oligosaccharides with a lower extent of sulfation as reported (3).
Product SourceCloned from Bacteroides Eggerthii and expressed in E. coli.
The following reagents are supplied with this product:
Store at (°C) Concentration Bacteroides Heparinase Reaction Buffer (10X) -20 10 X
- Product Categories:
- Depolymerization of Heparin/HS
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at email@example.com or 1-800-632-5227 x 8.