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Home PCR, qPCR & Amplification Technologies Products 3´-Desthiobiotin-GTP

3´-Desthiobiotin-GTP

3'-Desthiobiotin-GTP or 3'-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1).

Catalog #	Concentration	Size	List Price	Your Price
N0761S	5 mM	0.5 µmol	$333.00	Sign In Sign In

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Product Information
Supplied in: Ultrapure Water as a Triethylammonium salt at pH 7.0
Molecular Formula: C₃₁H₅₂N₁₁O₁₉P₃
Molecular Weight: 975.74 g/mol
Extinction Coefficient: λ₂₆₀ = ~11,700 Lmol-1 cm-1

This product is related to the following categories:

Nucleotide Solutions Products,

RNA Synthesis Products

This product can be used in the following applications:

RNA Modification,

In vitro Transcription for RNA Synthesis,

PCR
- Reagents Supplied
  Reagents Supplied
  
  The following reagents are supplied with this product:
  
  NEB # Component Name Component # Stored at (°C) Amount Concentration
  
  N0761S -20
  
  3´-Desthiobiotin-GTP N0761SVIAL -20 1 x 0.1 ml 5 mM
- Properties & Usage
- Related Products
  Companion Products
  
  Hydrophilic Streptavidin Magnetic Beads
  
  Vaccinia Capping System
- References
  Ettwiller, L. et al. (2016). BMC Genomics. 17, 199.
Protocols, Manuals & Usage
- Protocols
  Cappable-seq for prokaryotic transcription start site (TSS) determination
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N0761S_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N0761S_v1_10018146
  
  N0761S_v1_10033109
  
  N0761S_v1_10039547
  
  N0761S_v1_10052316
  
  N0761S_v1_10112959
  
  N0761S_v1_10133504
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  3´-Desthiobiotin-GTP
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.