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Home PCR, Polymerases & Amplification Technologies Products 3´-Desthiobiotin-GTP

3´-Desthiobiotin-GTP

Cappable-seq is a method, developed at NEB, for directly enriching the 5´ ends of primary transcripts. The method enables determination of transcription start sites at single base resolution (1). This is achieved by capping the 5´ triphosphorylated end of RNA with Vaccinia Capping System (NEB #M2080) and 3´-Desthiobiotin-GTP. The primary transcripts are then bound to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421), washed and eluted with free biotin.

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N0761S_pdp_800x450
  • 5 mM
    0.5 μmol
    $304.00
  • Product Information




    Supplied in: Ultrapure Water as a Triethylammonium salt at pH 7.0
    Molecular Formula: C31H52N11O19P3
    Molecular Weight: 975.74 g/mol
    Extinction Coefficient: λ260 = ~11,700 Lmol-1 cm-1
    Product Categories:
    Nucleotide Solutions,
    RNA Synthesis
    Applications:
    RNA Modification,
    In vitro Synthesis (IVT),
    PCR
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      The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
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      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

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      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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