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Home PCR, Polymerases & Amplification Technologies Products 3´-Desthiobiotin-GTP

3´-Desthiobiotin-GTP

Cappable-seq is a method, developed at NEB, for directly enriching the 5´ ends of primary transcripts. The method enables determination of transcription start sites at single base resolution (1). This is achieved by capping the 5´ triphosphorylated end of RNA with Vaccinia Capping System (NEB #M2080) and 3´-Desthiobiotin-GTP. The primary transcripts are then bound to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421), washed and eluted with free biotin.

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N0761S_pdp_800x450
  • 5 mM
    0.5 μmol
    $304.00
  • Product Information




    Supplied in: Ultrapure Water as a Triethylammonium salt at pH 7.0
    Molecular Formula: C31H52N11O19P3
    Molecular Weight: 975.74 g/mol
    Extinction Coefficient: λ260 = ~11,700 Lmol-1 cm-1
    Product Categories:
    Nucleotide Solutions,
    RNA Synthesis
    Applications:
    RNA Modification,
    In vitro Synthesis (IVT),
    PCR
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      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

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      For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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