3´-Desthiobiotin-GTP

3'-Desthiobiotin-GTP or 3'-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1).

Catalog #	Concentration	Size	List Price	Your Price
N0761S	5 mM	0.5 µmol	$323.00	Sign In Sign In

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Product Information
Supplied in: Ultrapure Water as a Triethylammonium salt at pH 7.0
Molecular Formula: C₃₁H₅₂N₁₁O₁₉P₃
Molecular Weight: 975.74 g/mol
Extinction Coefficient: λ₂₆₀ = ~11,700 Lmol-1 cm-1
Reagents Supplied

The following reagents are supplied with this product:
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NEB # Component Name Component # Stored at (°C) Amount Concentration

N0761S -20

3´-Desthiobiotin-GTP N0761SVIAL -20 1 x 0.1 ml 5 mM
Product Categories:

Nucleotide Solutions Products,

RNA Synthesis Products

Applications:

RNA Modification,

In vitro Synthesis (IVT),

PCR
- Properties & Usage
- Related Products
  Companion Products
  
  Hydrophilic Streptavidin Magnetic Beads
  
  Vaccinia Capping System
- References
  Ettwiller, L. et al. (2016). BMC Genomics. 17, 199.
Protocols, Manuals & Usage
- Protocols
  Cappable-seq for prokaryotic transcription start site (TSS) determination
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N0761Datasheet-Lot0011610
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N0761S_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N0761S_v1_10018146
  
  N0761S_v1_10033109
  
  N0761S_v1_10039547
  
  N0761S_v1_10052316
  
  N0761S_v1_10112959
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  3´-Desthiobiotin-GTP
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N0761Datasheet-Lot0011610
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.