Quick-Load^® 1 kb Plus DNA Ladder

This product was previously named the Quick-Load^® 2-Log DNA Ladder. There have been no changes to product formulation or specifications.This product is available in Quick-Load Purple format - enjoy sharper bands, no UV shadow, and a lower price.

Size range: 100 bp to 10 kb
Convenient, ready-to-load format
Formulated with bromophenol blue dye
Easy to identify reference bands
Suitable for 250 gel lanes

Catalog #	Concentration	Size	List Price	Your Price
N0469S	100 µg/ml	250 gel lanes (1.25 ml)	$83.00	Sign In Sign In

Please enter a quantity for at least one size

Product Information

Quick-Load® 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

NEB also offers a Quick-Load version of this ladder with purple dye.

Recommended gel percentage range: 1-1.4%
Optimum separation on 1.2%

This product is related to the following categories:: DNA Markers & Ladders Products

This product can be used in the following applications:: DNA Analysis

Reagents Supplied
Reagents Supplied

The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration

N0469S 4

Quick-Load^® 1 kb Plus DNA Ladder N0469SVIAL 4 1 x 1.25 ml 100 µg/ml

Properties & Usage

Bases

Fragment	Mass (ng)	bp
1	40	10,002
2	40	8,001
3	48	6,001
4	40	5,001
5	32	4,001
6	120	3,001
7	40	2,017
8	57	1,517
9	45	1,200
10	122	1,000
11	34	900
12	31	800
13	27	700
14	23	600
15	124	500 / 517
16	49	400
17	37	300
18	32	200
19	61	100

Effective Size Range

100bp to 10,002bp

Storage Buffer

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8 @ 25°C

Related Products
Companion Products
Product Notes
1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
2. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load 1 kb Plus DNA Ladder per gel lane.
3. In general, it is recommended to load 1µl per mm of gel lane.
4. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
5. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
6. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
References
1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual,(2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.

Protocols, Manuals & Usage
- Protocols
  Protocol for Quick-Load 1 kb Plus DNA Ladder (NEB #N0469)
Tools & Resources
- Selection Charts
  DNA Markers & Ladders Selection Tool
FAQs & Troubleshooting
- FAQs
  Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  
  How can I quantify the amount of DNA in each band of a marker?
  
  Can I use Midori Green with the DNA Ladders from NEB?
  
  Can I use SYBR^® and/or GelRed^® dyes with the DNA Ladders from NEB?
  
  Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
  
  What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?
- Tech Tips
  
  Mix well before use, especially after thawing.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N0469S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N0469S_L_v1_0211403
  
  N0469S_L_v1_0221407
  
  N0469S_L_v1_0231412
  
  N0469S_L_v1_0231503
  
  N0469S_L_v1_0231504
  
  N0469S_L_v1_0241505
  
  N0469S_L_v1_0251506
  
  N0469S_L_v1_0251509
  
  N0469S_L_v1_0261511
  
  N0469S_L_v1_0261512
  
  N0469S_L_v1_0251510
  
  N0469S_L_v1_0271604
  
  N0469S_L_v1_0271608
  
  N0469S_L_v1_0271612
  
  N0469S_L_v1_0271701
  
  N0469S_L_v1_0281704
  
  N0469S_L_v1_0291711
  
  N0469S_v2_10008158
  
  N0469S_v2_10030379
  
  N0469S_v2_10035524
  
  N0469S_v2_10055591
  
  N0469S_v2_10059940
  
  N0469S_v2_10064764
  
  N0469S_v2_10076401
  
  N0469S_v2_10080160
  
  N0469S_v2_10083314
  
  N0469S_v2_10087052
  
  N0469S_v2_10100076
  
  N0469S_v2_10103921
  
  N0469S_v2_10122996
  
  N0469S_v2_10148784
  
  N0469S_v2_10162285
  
  N0469S_v2_10171418
  
  N0469S_v2_10176804
  
  N0469S_v2_10182372
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Quick-Load^® 1 kb Plus DNA Ladder
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Trademarks
  QUICK-LOAD® is a registered trademark of New England Biolabs, Inc.
  FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.

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