Bases

Fragment	Mass	bp
1	40	10,002
2	40	8,001
3	48	6,001
4	40	5,001
5	32	4,001
6	120	3,001
7	40	2,017
8	57	1,517
9	45	1,200
10	122	1,000
11	34	900
12	31	800
13	27	700
14	23	600
15	124	500 / 517
16	49	400
17	37	300
18	32	200
19	61	100

Effective Size Range

100bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

Companion Products

• Quick-Load® 100 bp DNA Ladder
• Quick-Load® 1 kb DNA Ladder
• 100 bp DNA Ladder
• 1 kb DNA Ladder
• 50 bp DNA Ladder
• TriDye™ 1 kb Plus DNA Ladder
• TriDye™ 100 bp DNA Ladder
• TriDye™ 1 kb DNA Ladder
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
2. In general, it is recommended to load 1µl per mm of gel lane.
3. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
4. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.

1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
2. How can I quantify the amount of DNA in each band of a marker?
3. Can I use GelRed® with the DNA Ladders from NEB?
4. Can I use Midori Green with the DNA Ladders from NEB?
5. Can I use SYBR® with the DNA Ladders from NEB?

• DNA Markers & Ladders Selection Chart

• NEBioCalculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N0469S_L_v1
• N0469S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N0469S_L_v1_0211403
• N0469S_L_v1_0221407
• N0469S_L_v1_0231412
• N0469S_L_v1_0231503
• N0469S_L_v1_0231504
• N0469S_L_v1_0241505
• N0469S_L_v1_0251506
• N0469S_L_v1_0251509
• N0469S_L_v1_0261511
• N0469S_L_v1_0261512
• N0469S_L_v1_0251510
• N0469S_L_v1_0271604
• N0469S_L_v1_0271608
• N0469S_L_v1_0271612
• N0469S_L_v1_0271701
• N0469S_L_v1_0281704
• N0469S_L_v1_0291711
• N0469S_v2_10008158

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Quick-Load^® 1 kb Plus DNA Ladder

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Trademarks

QUICK-LOAD® is a registered trademark of New England Biolabs, Inc.
FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.