Home Markers & Ladders Products Quick-Load^® 1 kb Plus DNA Ladder

Quick-Load^® 1 kb Plus DNA Ladder

This product was previously named the Quick-Load^® 2-Log DNA Ladder. There have been no changes to product formulation or specifications.This product is available in Quick-Load Purple format - enjoy sharper bands, no UV shadow, and a lower price.

Size range: 100 bp to 10 kb
Convenient, ready-to-load format
Formulated with bromophenol blue dye
Easy to identify reference bands
Suitable for 250 gel lanes

Ordering Information

Catalog #

Concentration

100 μg/ml

Size

1.25 ml

List Price

$74.00

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Product Information

Quick-Load® 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

NEB also offers a Quick-Load version of this ladder with purple dye.

Recommended gel percentage range: 1-1.4%
Optimum separation on 1.2%

Product Categories:: DNA Markers & Ladders

Applications:: DNA Analysis

Properties and Usage

Bases

Fragment	Mass	bp
1	40	10,002
2	40	8,001
3	48	6,001
4	40	5,001
5	32	4,001
6	120	3,001
7	40	2,017
8	57	1,517
9	45	1,200
10	122	1,000
11	34	900
12	31	800
13	27	700
14	23	600
15	124	500 / 517
16	49	400
17	37	300
18	32	200
19	61	100

Effective Size Range

100bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

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Notes
1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
2. In general, it is recommended to load 1µl per mm of gel lane.
3. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
4. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
6. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load 1 kb Plus DNA Ladder per gel lane.
References
1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.

FAQs & Tech Tips
- FAQs
  Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  
  How can I quantify the amount of DNA in each band of a marker?
  
  Can I use GelRed with the DNA Ladders from NEB?
  
  Can I use Midori Green with the DNA Ladders from NEB?
  
  Can I use SYBR® with the DNA Ladders from NEB?
- Tech Tips
  
  Mix well before use, especially after thawing.
Protocols & Manuals
- Protocols
  Protocol for Quick-Load 1 kb Plus DNA Ladder (NEB #N0469)
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N0469Datasheet-Lot0171204
  
  N0469Datasheet-Lot0171209
  
  N0469Datasheet-Lot0191302
Other Tools & Resources
- Selection Charts
  DNA Markers & Ladders Selection Chart
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N0469S_L_v1
  
  N0469S_L_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N0469S_L_v1_0211403
  
  N0469S_L_v1_0221407
  
  N0469S_L_v1_0231412
  
  N0469S_L_v1_0231503
  
  N0469S_L_v1_0231504
  
  N0469S_L_v1_0241505
  
  N0469S_L_v1_0251506
  
  N0469S_L_v1_0251509
  
  N0469S_L_v1_0261511
  
  N0469S_L_v1_0261512
  
  N0469S_L_v1_0251510
  
  N0469S_L_v1_0271604
  
  N0469S_L_v1_0271608
  
  N0469S_L_v1_0271612
  
  N0469S_L_v1_0271701
  
  N0469S_L_v1_0281704
  
  N0469S_L_v1_0291711
  
  N0469S_v2_10008158
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Quick-Load^® 1 kb Plus DNA Ladder
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N0469Datasheet-Lot0171204
  
  N0469Datasheet-Lot0171209
  
  N0469Datasheet-Lot0191302
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  Trademarks
  QUICK-LOAD® is a registered trademark of New England Biolabs, Inc.
  FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.

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