Ribonucleotide Solution Set

Description

The Ribonucleotide Solution Set (NTP Set) consists of four separate solutions of ATP, GTP, CTP and UTP, pH 7.5 as sodium salts. The NTPs are also available as a Ribonucleotide Solution Mix (NEB #N0466).

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
ATP -20100 mM
GTP -20100 mM
UTP -20100 mM
CTP -20100 mM

Advantages and Features

Applications

In vitro transcription
RNA tailing by Poly(A) and Poly(U) Polymerases
Substrates or cofactors for many enzymes

Properties and Usage

Storage Temperature

-20°C

Notes

  1. Store at -20°C. Avoid multiple freeze/thaw cycles. Dilute with nuclease-free water when necessary. NTPs are used with many RNA products from NEB.

Tech Tips

It is important to vortex the tubes prior to use due to high concentration of the nucleotides.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Assurance Statement

  • NTPs are highly purified, > 99% pure by HPLC, and are free from detectable DNase, RNase and Nickase. They were validated by in vitro transcription using the T7 High Yield RNA Synthesis Kit (NEB #E2040).

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 16 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Physical Purity (HPLC):
    The purity of the product is determined by HPLC analysis.
  • RNase Activity (16 Hour Digestion):

    The product is tested in a reaction containing a RNA substrate.  After incubation for 16 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.