EnGen® Lba Cas12a (Cpf1)

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EnGen Lba Cas12a (Cpf1) is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Targeting requires a gRNA complementary to the target site as well as a 5' TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends.

  • T-rich TTTN PAM sequence opens up additional genomic regions for targeting
  • Shorter, 40-44 base guide RNA
  • Two nuclear localization signals for improved transport to the nucleus
  • 5’ overhanging termini on cleavage products
  • Active from 16 to 48°C
  • Maintains activity at lower temperatures than the Acidaminococcus orthologs, permitting editing in ectothermic organisms such as zebra fish and xenopus
  • High concentration liquid format can be used for microinjection, electroporation and lipofection

Ordering Information

M0653T_pdp_800X450
  • 1 μM
    70 pmol
    $70.00
  • 100 μM
    2,000 pmol
    $250.00
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