FAQ: What are the differences between EnGen® Lba Cas12a (Cpf1) (NEB #M0653) and EnGen Spy Cas9 NLS (NEB #M0646)?

There are a number of differences between EnGen Lba Cas12a (Cpf1) and EnGen Spy Cas9 NLS. These differences are summarized in the table below.

  EnGen Spy Cas9 NLS
EnGen Lba Cas12a (Cpf1)
Classification Class II Type II Class II Type V
Nuclease Domains RuvC and HNH RuvC only
Size 161.3 kDa 150.9 kDa
Guide RNA Length ~100 nt ~41-44 nt
Cleavage Site 3 bases 5´ of the PAM ~18 bases 3´ of the PAM
Termini of Cleaved Product Blunt ends 5´ overhang