There are a number of differences between EnGen Lba Cas12a (Cpf1) and EnGen Spy Cas9 NLS. These differences are summarized in the table below.
| EnGen Spy Cas9 NLS |
EnGen Lba Cas12a (Cpf1) | |
| Classification | Class II Type II | Class II Type V |
| Nuclease Domains | RuvC and HNH | RuvC only |
| Size | 161.3 kDa | 150.9 kDa |
| Guide RNA Length | ~100 nt | ~41-44 nt |
| PAM Sequence | NGG | TTTN |
| Cleavage Site | 3 bases 5´ of the PAM | ~18 bases 3´ of the PAM |
| Termini of Cleaved Product | Blunt ends | 5´ overhang |
