NEBNext® Ultra™ II Q5® Master Mix

NEBNext Ultra II Q5® Master Mix is the most recent NEBNext formulation of Q5 High Fidelity DNA Polymerase, optimized for amplification of NGS libraries. This hot start formulation is designed for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, and further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.

NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.

  • Optimized for high yields in NGS library amplification
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification with Q5, the highest-fidelity polymerase (1)
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step

Ordering Information

  • 50 reactions
  • 250 reactions
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