HomeFAQsCan I use NEBNext Ultra II Q5 Master Mix to amplify bisulfite-converted libraries?
FAQ: Can I use NEBNext Ultra II Q5 Master Mix to amplify bisulfite-converted libraries?
No. NEBNext Ultra II Q5 Master Mix contains a family B-type DNA polymerase that cannot tolerate uracil/dUTP. NEBNext Ultra II Q5 Master Mix is hence not compatible bisulfite-converted DNAs and cannot incorporate dUTP. For protocols employing bisulfite-treated DNA, EpiMark™ Hot Start DNA Polymerase can be used.
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