HomeFAQsWhat is the difference between NEBNext Ultra II Q5 Master Mix and NEBNext High-Fidelity 2X PCR Master Mix (M0541)?
FAQ: What is the difference between NEBNext Ultra II Q5 Master Mix and NEBNext High-Fidelity 2X PCR Master Mix (M0541)?
Although both master mixes contain Q5 High-Fidelity DNA Polymerase, the master mix composition is different. NEBNext Ultra II Q5 Master Mix has been specifically optimized for to faithfully amplify NGS libraries with the least amount of amplification bias. Additionally, amplification of NGS libraries in the presence or absence of magnetic beads is more robust, and it is a hot start, making it ideal for workflows involving automation.
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