Bst 2.0 WarmStart® DNA Polymerase
Optimized for Loop-Mediated Isothermal Amplification (LAMP)
- Consistent amplification performance with the convenience of room temperature setup
- WarmStart® technology eliminates off-target amplifilcation and offers increased reaction efficiency
- Optimal reaction performance from 60-72 °C
Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
Product SourceBst 2.0 WarmStart DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter.
The following reagents are supplied with this product:
Store at (°C) Concentration Isothermal Amplification Buffer Pack -20 10X Magnesium Sulfate (MgSO4) Solution -20 100 mM
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