Features

 

Application Features

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X LongAmp™ Taq Reaction Buffer

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

Yes

Strand Displacement

+

Unit Assay Conditions

1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.

Companion Products

• Magnesium Sulfate (MgSO₄) Solution
• LongAmp® Taq PCR Kit
• LongAmp® Taq 2X Master Mix
• LongAmp® Taq DNA Polymerase
• LongAmp® Hot Start Taq 2X Master Mix
• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

Materials Sold Separately

• LongAmp® Taq Reaction Buffer

1. 5'→3' flap endonuclease destroys displaced strand.

1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. What is the recommended enzyme amount when using LongAmp™ Hot Start Taq DNA Polymerase?
2. What is the fidelity of the LongAmp™ Hot Start Taq DNA Polymerase compared to Taq DNA Polymerase?
3. Can the extension step be carried out at 72°C when using LongAmp™ Hot Start Taq DNA Polymerase?
4. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Hot Start Taq DNA Polymerase?
5. What is the extension rate when using LongAmp™ Hot Start Taq DNA Polymerase?
6. Why is the product a smear when visualized on an agarose gel?
7. Can LongAmp™ Hot Start Taq DNA Polymerase be used to amplify GC-rich amplicons?

1. PCR Protocol for LongAmp^® Hot Start Taq DNA Polymerase (M0534)

• DNA Polymerase Selection Chart
• PCR Selection Chart
• Thermophilic DNA Polymerases

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

• NEBioCalculator
• PCR Selector
• Tm Calculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0534S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0534S_L_v1_0061512
• M0534S_L_v1_0071606
• M0534S_L_v1_0071612
• M0534S_L_v1_0071712

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• LongAmp^® Hot Start Taq DNA Polymerase
• LongAmp^® Taq Reaction Buffer Pack

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

Notice to Purchaser:
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based LongAmp Hot Start Taq DNA Polymerase for RESEARCH PURPOSES ONLY. Commercial use of the aptamer-based LongAmp Hot Start Taq DNA Polymerase requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.

Trademarks

LONGAMP^® and THERMOPOL^® are registered trademarks of New England Biolabs, Inc.
DEEP VENT™ is a trademark of New England Biolabs, Inc.

IGEPAL^® is a registered trademark of Rhodia Operations.
TWEEN^® is registered trademark of Uniqema Americas LLC.