LongAmp® Hot Start Taq DNA Polymerase
LongAmp® Hot Start Taq DNA Polymerase offers exceptional performance with long amplicons.
- Aptamer-based inhibitor enables room temperature reaction setup
- Optimized enzyme blend for amplification up to 30 kb
- 2X higher fidelity than Taq
- Master mix version available
Featured Videos
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Tips for Amplifying Large Amplicons
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Why is Tm Important in Primer Design?
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5 Tips for Setting Up Your PCR
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Overview of PCR
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Product Information
LongAmp Hot Start Taq DNA Polymerase is a unique blend of aptamer-based Hot Start Taq and Deep VentR™ DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The 3´→5´ exonuclease activity of Deep VentR DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase (1). LongAmp Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.
Amplification of a 6 kb amplicon from varying amounts of human genomic DNA using LongAmp Hot Start Taq DNA Polymerase. Starting template amounts are indicated below the gel. Marker M is the NEB 2-Log DNA Ladder (NEB #N3200) Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration LongAmp® Taq Reaction Buffer Pack -20 5 X - Product Categories:
- LongAmp® DNA Polymerases
- Applications:
- Hot Start PCR,
- Long Range PCR,
- Specialty PCR ,
- Routine PCR ,
- PCR
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