LongAmp^® Hot Start Taq DNA Polymerase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0534S     -20       LongAmp® Hot Start Taq DNA Polymerase M0534SVIAL -20 1 x 0.2 ml 2,500 units/ml   LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 1 x 1.5 ml 5 X
M0534L     -20       LongAmp® Hot Start Taq DNA Polymerase M0534LVIAL -20 1 x 1 ml 2,500 units/ml   LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 4 x 1.5 ml 5 X

Features

 

Application Features

 

Companion Products

• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix
• LongAmp® Taq PCR Kit
• Magnesium Sulfate (MgSO₄) Solution
• LongAmp® Taq DNA Polymerase
• LongAmp® Hot Start Taq 2X Master Mix
• LongAmp® Taq 2X Master Mix

Materials Sold Separately

• LongAmp® Taq Reaction Buffer

1. 5'→3' flap endonuclease destroys displaced strand.

1. Barnes, W.M. (1994). Proc.Natl. Acad. Sci. USA. 91, 2216-2220.
2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

1. PCR Protocol for LongAmp® Hot Start Taq DNA Polymerase (M0534)

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart
• Thermophilic DNA Polymerases

• PCR Selector
• Tm Calculator

1. What is the recommended enzyme amount when using LongAmp™ Hot Start Taq DNA Polymerase?
2. What is the fidelity of the LongAmp™ Hot Start Taq DNA Polymerase compared to Taq DNA Polymerase?
3. Can the extension step be carried out at 72°C when using LongAmp™ Hot Start Taq DNA Polymerase?
4. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Hot Start Taq DNA Polymerase?
5. What is the extension rate when using LongAmp™ Hot Start Taq DNA Polymerase?
6. Why is the product a smear when visualized on an agarose gel?
7. Can LongAmp™ Hot Start Taq DNA Polymerase be used to amplify GC-rich amplicons?
8. Is Monarch-purified gDNA suitable for long-range PCR?

• PCR Troubleshooting Guide
• Taq PCR Kit Troubleshooting Guide

• Using aptamers to control enzyme activities: Hot Start Taq and beyond

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0534S_L_v1
• M0534S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0534S_L_v1_0061512
• M0534S_L_v1_0071606
• M0534S_L_v1_0071612
• M0534S_L_v1_0071712
• M0534S_v1_10015647
• M0534L_v1_10016146
• M0534S_v1_10024742
• M0534L_v1_10029420
• M0534L_v1_10037245
• M0534S_v1_10048518
• M0534L_v1_10048517
• M0534S_v1_10060285
• M0534L_v2_10069476
• M0534S_v2_10074203
• M0534L_v2_10075651
• M0534S_v2_10099468
• M0534L_v2_10108227
• M0534S_v2_10109943
• M0534L_v2_10117357
• M0534L_v2_10122834
• M0534S_v2_10125365
• M0534L_v2_10140976
• M0534S_v2_10138865

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• LongAmp^® Hot Start Taq DNA Polymerase

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the LongAmp^® Hot Start Taq DNA Polymerase for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com. 

Trademarks

LONGAMP^® and THERMOPOL^® are registered trademarks of New England Biolabs, Inc.
DEEP VENT^® is a trademark of New England Biolabs, Inc.
IGEPAL^® is a registered trademark of Rhodia Operations.
TWEEN^® is registered trademark of Uniqema Americas LLC.