Home DNA Modifying Enzymes and Cloning Technologies Products Msz Exonuclease I

Msz Exonuclease I
neb31 55 80 Heat

M0527 Mechanism

  • DNA-specific exonuclease
  • Can be heat inactivated at 80°C in 1 minute
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Msz Exonuclease I (MszExoI) is Ideal for: 

  • Removal of linear single-stranded DNA oligos in thermophilic workflow applications within 45°C and 60°C.

  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
 

Featured Video

Exo_Intro_Anim_Thumb_720

What are exonucleases and their applications?

View additional videos
Catalog # Concentration Size List Price Your Price Quantity
M0527S 10,000 units/ml 1,000 units $78.00
Please enter a quantity for at least one size
Need a custom/large volume order? Contact us
  • Msz Exonuclease I is isolated from a strain of E. coli, which carries the cloned pMC45-1 gene from Methylocaldum szegediense. Msz Exonuclease I is a DNA specific exonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction with optimal activity between 45°C and 60°C in rCutSmart™ Buffer. 
    This product is related to the following categories:
    Exonucleases and Non-specific Endonucleases Products

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit of Msz Exonuclease I is defined as the amount of enzyme that will catalyze the release of 5 nmol of acid-soluble nucleotide in a total reaction volume of 100 µl in 15 minutes at 55°C in 1X rCutSmart Buffer.

      Reaction Conditions

      1X rCutSmart™ Buffer
      Incubate at 55°C

      1X rCutSmart™ Buffer
      50 mM Potassium Acetate
      20 mM Tris-acetate
      10 mM Magnesium Acetate
      100 µg/ml Recombinant Albumin
      (pH 7.9 @ 25°C)

      Heat Inactivation

      80°C for 1 minute

    • Related Products

      Companion Products

    • References

      1. Fei L, Tian S, Moysey R, Misca M, Barker JJ, Smith MA, et al. (2015). Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense. PLoS ONE. 10 (2), e0117470. DOI: 10.1371/journal.pone.0117470

  • Protocols, Manuals & Usage

  • Tools & Resources

  • FAQs & Troubleshooting

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      Effective 9-5-23, unit definition changed.
       
      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Featured Videos

View Video Library

Other Products You May Be Interested In

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.

Continue

Choose your country

North America

Europe

Asia-Pacific

If you don't see your country above, please visit our international site

animated golden butterfly flying around

Find a Golden Butterfly for a Chance to Win!

Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.

Learn More
Remind me next time

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.