Enzymes for Innovation (EFI)
Products Available
| 5-hydroxymethyluridine DNA Kinase | M0659 |  5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA. |
| Boletopsis grisea Lectin (BGL) |
P0867 |
Enables enrichment of GlcNAc-capped N-glycans or mucin type O-glycopeptides from complex samples in glycomics and glycoproteomics analytical workflows. |
| Endoglycoceramidase I (EGCase I) | P0773 |  Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids. |
| 9°N™ Reverse Gyrase | M0200 |  9°N™ Reverse Gyrase is a thermostable Type IA topoisomerase that introduces positive supercoils into double-stranded closed-circular DNA in the presence of ATP (1). |
| FTO RNA Demethylase | M0616 |  Catalyzes the demethylation of N6-methyladenosine (m6A) on RNA to form adenosine. |
| Immobilized T4 DNA Ligase |
M0569 |
Immobilized T4 DNA Ligase is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, the enzyme can be removed using a magnet and can be re-used. |
| MszExonuclease I (Msz Exol) |
M0527 |  Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction with optimal activity between 45°C and 60°C. |
| Nb.BssSI | R0681 |  Generates a nick in the 3´ strand at the following recognition site: CACGAG (none/-1). |
| NudC Pyrophosphatase | M0607 |
NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+ - and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding). |
| RtcB Ligase | M0458 |  Joins single-stranded RNA with a 3´-phosphate or 2´,3´-cyclic phosphate to another RNA with a 5´ -hydroxyl. |
| Sce PUS1 |
M0526 |
Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer. |
| Tte UvrD Helicase | M1202 |
Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. |
| Tth Argonaute | M0665 |
TtAgo is a prokaryotic argonaute that functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5´-phosphorylated single-stranded DNA oligonucleotide guide. |
References: 1. Lulchev,P., and Klostermeier, D. (2014), NAR 42(13), 8200-8213.
Products Available
| 5-hydroxymethyluridine DNA Kinase | M0659 | 5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA. |
| EcoGII Methyltransferase | M0603 |  EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context. |
| Endoglycoceramidase I (EGCase I) | P0773 | Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids. |
| Immobilized T4 DNA Ligase |
M0569 |
Immobilized T4 DNA Ligase is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, the enzyme can be removed using a magnet and can be re-used. |
| Nb.BssSI | R0681 | Generates a nick in the 3´ strand at the following recognition site: CACGAG (none/-1). |
| NudC Pyrophosphatase | M0607 |
NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+ - and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding). |
| RtcB Ligase | M0458 | Joins single-stranded RNA with a 3´-phosphate or 2´,3´-cyclic phosphate to another RNA with a 5´ -hydroxyl. |
| Sce PUS1 |
M0526 |
Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer. |
| TelN Protelomerase | M0651 |  Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage. |
| Thermostable FEN1 | M0645 |  Catalyzes the cleavage of 5´ DNA flaps from branched double-stranded DNA substrates. |
| Tte UvrD Helicase | M1202 |
Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. |
| Tth Argonaute | M0665 |
TtAgo is a prokaryotic argonaute that functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5´-phosphorylated single-stranded DNA oligonucleotide guide. |
If you are looking for an enzyme functionality that is not currently available, contact EnzymesForInnovation@neb.com.
Have you found a good application for one of the enzymes listed above? We would love to hear about it!
