Apyrase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0398S     -20       Apyrase M0398SVIAL -20 1 x 0.02 ml 500 units/ml   Apyrase Reaction Buffer B0398SVIAL -20 1 x 1.25 ml 10 X
M0398L     -20       Apyrase M0398LVIAL -20 1 x 0.1 ml 500 units/ml   Apyrase Reaction Buffer B0398SVIAL -20 1 x 1.25 ml 10 X

Features

• Enzyme of highest purity, efficiency and quality 
• Tested in updated range of more stringent quality controls
• Supplied at 10-fold higher concentration in the optimized storage buffer
• Supplied with new optimal reaction buffer
• Provided with updated, detailed characterization
• Discovered and characterized a new function: the ability to convert 5’ triphosphorylated RNA to monophosphorylated form, which can be ligated or removed by 5’ exonuclease

Application Features

• Highly efficient degradation of ATP to AMP.
• Removal of deoxynucleotides in DNA pyrosequencing between cycles (3).
• Conversion of 5´ triphosphorylated RNA to ligatable monophosphorylated form that can be used for 5´ RNA adaptor ligation.
• Conversion of 5´ triphosphorylated RNA to 5´ exonuclease XRN-1 (NEB #M0338) sensitive monophosphorylated RNA.

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Apyrase has a higher ratio of activity for ATP:ADP (14:1).
2. Apyrase is a calcium-activated enzyme. It is approximately 50% active when Mg²⁺ substitutes Ca²⁺ in Apyrase Reaction Buffer.
3. As a metal-dependent enzyme, Apyrase can be inhibited by EGTA and EDTA.
4. The activity of Apyrase is approximately 30% in NEBuffers r1.1, r2.1, r3.1 and rCutSmart™ Buffer.
5. Apyrase does not remove 5´ caps from eukaryotic mRNA.

1. Kettlun, A.M. et al. (2005). Phytochemistry. 66, 975-982.
2. Schildkraut, I., Schildkraut, E. and Barshevsky, T., New England Biolabs, Inc. Unpublished observation
3. Hornung, V. et al . (2006). Science. 314, 994-997.
4. Schildkraut, I. and Schildkraut, E., New England Biolabs, Inc. Unpublished observation

1. What is the activity of Apyrase in other NEBuffers?
2. Can Mg²⁺ be substituted for Ca²⁺ in Apyrase reaction buffer?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0398S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0398S_L_v1_0011610
• M0398S_L_v1_0011703
• M0398S_L_v1_0011709
• M0398S_L_v1_0011804
• M0398L_v1_10016796
• M0398S_v1_10019357
• M0398L_v1_10027502
• M0398S_v1_10027503
• M0398L_v1_10029145
• M0398S_v1_10029146
• M0398L_v1_10041754
• M0398L_v1_10049222
• M0398S_v1_10041756
• M0398L_v1_10053931
• M0398S_v1_10053930
• M0398L_v1_10057999
• M0398S_v1_10057998
• M0398L_v1_10067999
• M0398L_v1_10074603
• M0398L_v1_10082473
• M0398S_v1_10082475
• M0398L_v1_10092632
• M0398S_v1_10105439
• M0398S_v1_10121850
• M0398L_v1_10092633
• M0398L_v1_10126955
• M0398S_v1_10126957
• M0398L_v1_10149232
• M0398L_v1_10154760
• M0398S_v1_10149234
• M0398L_v1_10162923
• M0398S_v1_10162925

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Apyrase
• Apyrase Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.