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  • Adenosine 5'-Triphosphate (ATP)

    Description

    Adenosine 5´-Triphosphate (ATP) is a substrate for many ATP-dependent enzyme systems (1). 

    Product Source

    Isolated from a microbial source.

    Properties and Usage

    Storage Temperature

    -20°C

    Molecular Weight

    Theoretical: 551.2 daltons

    Storage Notes

    • For long term storage (>30 days), store at -70°C.

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 16 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Exonuclease Activity (Radioactivity Release):
      The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
    • Non-Specific RNase Activity (16 hour, RNA):
      The RNA is tested in a reaction under standard reaction conditions. After incubation for 16 hours there is no detectable degradation of the RNA as determined by agarose gel electrophoresis.
    • Phosphatase Activity (Oligo):
      The product is tested in a reaction containing a 5'-phosphorylated oligo. After incubation there is no detectable dephosphorylation as determined by SDS-PAGE
    • Protease Activity (SDS-PAGE):

      The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable degradation of the proteins as determined by SDS-PAGE.

    Notes

    1. Storage: Sterile purified water adjusted to pH 7.0 with NaOH.
    2. For long term storage, keep in small aliquots at -70°C.

    References

    1. Knowles, J.R. (1980). 49, 479-919. Annie Rev. Biochem..

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Usage Guidelines & Tips

    Troubleshooting Guides