Bsu DNA Polymerase, Large Fragment

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Optimal performance in a number of applications

  • Strand displacement DNA synthesis
  • Random primer labeling
  • Second strand cDNA synthesis

Ordering Information

  • 5,000 units/ml
    200 units
  • 5,000 units/ml
    1,000 units
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  • Product Information
    Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I (1), but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.

    Product Source

    An E. coli strain that contains a genetic fusion of the Bacillus subtilis DNA polymerase I gene (starting from codon 297 thus lacking the 5´→ 3´ exonuclease domain), and the gene coding for maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion is cleaved off in vitro. The remaining DNA polymerase is purified free of MBP.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBuffer™ 2 -20 10 X
    Product Categories:
    DNA Manipulation
    Polymerases for DNA Manipulation,
    Isothermal Amplification,
    RT-PCR & cDNA Synthesis
    RT-qPCR, RT-PCR and cDNA Synthesis
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