Taq 2X Master Mix

Description

  
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

Taq
2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

Highlights

  • Robust and reliable reactions
  • Tolerates a wide range of templates
  • Exceptional value in terms of cost per unit

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Magnesium Chloride (MgCl2) Solution-2025 mM

Advantages and Features

Applications

  • PCR
  • Primer Extension
  • Colony PCR

Properties and Usage

Storage Temperature

-20°C

1X Master Mix Composition

10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
0.2 mM dNTPs
5% Glycerol
0.08% IGEPAL® CA-630
0.05% Tween® 20
25 units/ml Taq DNA Polymerase
pH 8.6@25°C

Heat Inactivation

No

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

Notes

  1. Taq 2X Master Mix should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.
  2. Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C
  3. Taq 2X Master Mix is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.

FAQs

  1. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
  2. Why is the product a smear when visualized on an agarose gel?
  3. Why is there no product when visualized on an agarose gel?
  4. The product sequence doesn't completely match the expected sequence. How can this result be improved?
  5. Does the presence of Ca2+ inhibit PCR?
  6. What are the stability and storage requirements of the OneTaq® Master Mixes?

Tech Tips

Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

Protocols

  1. Protocol for Taq 2X Master Mix (M0270)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Troubleshooting Guides

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • DNase Activity (Labeled Oligo, 3' extension):
    The product is tested in a reaction containing a fluorescent  labeled double stranded oligonucleotide containing a 3' extension. The percent degradation is determined by capillary electrophoresis.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • PCR Amplification (Master Mix):
    The polymerase master mix is tested in a polymerase chain reaction (PCR) using a control template and specific primers, resulting in the expected product.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.