Endonuclease III (Nth)

Application Features

• Single cell gel electrophoresis (Comet assay) (3,4,5)
• Alkaline elution (6)
• Alkaline unwinding (7)

1. Dizdaroglu, M., Laval, J. and Boiteux, S. (1993). Biochemistry. 32, 12105-12111.
2. Hatahet, Z. et al. (1994). J. Biol. Chem.. 269, 18814-18820.
3. Singh, N. et al. (1988). Exp. Cell Res.. 175, 184-191.
4. Collins, A. et al. (1993). Carcinogenesis. 14, 1733-1735.
5. Collins, A. et al. (1996). Environ. Health Perspect . 104, 465-469.
6. Pflaum, M. et al. (1998). Free Rad. Res.. 29, 585-594.
7. Hartwig, A. et al. (1996). Toxicol. Lett. 88, 85-90.

1. Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)

• Activity of DNA Modifying Enzymes in CutSmart® Buffer
• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. What is the activity of Endonuclease III in the NEBuffer 1-4?
2. What is the molecular weight of Endonuclease III?
3. What types of damaged bases are recognized and removed by Endonuclease III?
4. Is Endonuclease III a tagged protein?
5. What substrate is used to test Endonuclease III activity?
6. Will Endonuclease III work in CutSmart buffer?

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The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0268S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0268S_v1_10011681
• M0268S_v1_10031989
• M0268S_v1_10047988
• M0268S_v1_10060392

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• Endonuclease III (Nth)

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