Endonuclease III (Nth)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0268S     -20       Endonuclease III (Nth) M0268SVIAL -20 1 x 0.1 ml 10,000 units/ml   Endonuclease III (Nth) Reaction Buffer B0268SVIAL -20 1 x 1 ml 10 X

Application Features

• Single cell gel electrophoresis (Comet assay) (3,4,5)
• Alkaline elution (6)
• Alkaline unwinding (7)

1. Effective with M0268S/L spec V2, NEB has updated the product specification as follows:
2. Protein Purity Assay (SDS-PAGE):  Endonuclease III (Nth) is ≥95% pure as determined by SDS PAGE analysis using Coomassie Blue detection.
3. qPCR DNA Contamination (E. coli Genomic):  A minimum of 1 μl of Endonuclease III (Nth) is screened for the presence of E. coli genomic DNA using SYBR^® Green qPCR with primers specific for the E. coli 16S rRNA locus.  Results are quantified using a standard curve generated from purified E. coli genomic DNA. The measured level of E. coli genomic DNA contamination is ≤1 E. coli genome.
4. Endonuclease Activity (Nicking): Removed to align with standard quality control testing for product portfolio.

1. Dizdaroglu, M., Laval, J. and Boiteux, S. (1993). Biochemistry. 32, 12105-12111.
2. Hatahet, Z. et al. (1994). J. Biol. Chem.. 269, 18814-18820.
3. Singh, N. et al. (1988). Exp. Cell Res.. 175, 184-191.
4. Collins, A. et al. (1993). Carcinogenesis. 14, 1733-1735.
5. Collins, A. et al. (1996). Environ. Health Perspect . 104, 465-469.
6. Pflaum, M. et al. (1998). Free Rad. Res.. 29, 585-594.
7. Hartwig, A. et al. (1996). Toxicol. Lett. 88, 85-90.

1. Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer
• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. What is the activity of Endonuclease III in the NEBuffer 1-4?
2. What is the molecular weight of Endonuclease III?
3. What types of damaged bases are recognized and removed by Endonuclease III?
4. Is Endonuclease III a tagged protein?
5. What substrate is used to test Endonuclease III activity?
6. Will Endonuclease III work in rCutSmart buffer?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0268S_L_v1
• M0268S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0268S_v1_10011681
• M0268S_v1_10031989
• M0268S_v1_10047988
• M0268S_v1_10060392
• M0268S_v1_10081417
• M0268S_v1_10088298
• M0268S_v1_10097290
• M0268S_v2_10133535
• M0268S_v2_10150676
• M0268S_v2_10167462
• M0268S_v2_10171166

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Endonuclease III (Nth)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.