Endonuclease III (Nth)
- Single-cell gel electrophoresis (Comet Assay)
- Alkaline elution
- Alkaline unwinding
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Endonuclease III (Nth) protein from E. coli acts as both N-glycosylase and a AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site). The AP-lyase activity of the enzyme cleaves 3´ to the AP site leaving a 5´ phosphate and a 3´ ring opened sugar. Some of the damaged bases recognized and removed by Endouclease III include urea, 5, 6 dihydroxythymine, thymine glycol, 5-hydroxy-5 methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihdrothimine and methyltartronylurea (1,2).
Product SourceAn E. coli strain which carries the cloned nth gene from Escherichia coli.
The following reagents are supplied with this product:
Store at (°C) Concentration Endonuclease III (Nth) Reaction Buffer -20 10 X
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