RecJ_f

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0264S     -20       RecJf M0264SVIAL -20 1 x 0.033 ml 30,000 units/ml   NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X
M0264L     -20       RecJf M0264LVIAL -20 1 x 0.165 ml 30,000 units/ml   NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• NEBuffer 2

1. Lovett, S.T., Kolodner, R.D. (1989). Proc. Natl. Acad. Sci. USA. 86, 2627-2631.
2. Lovett, S. and Whitaker, R. Unpublished observation
3. Whitaker, R. Unpublished observation

1. Protocol for RecJ_f (NEB #M0264)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

1. What is the activity of RecJ_f in the NEBuffers?
2. Can DNA be blunted using RecJ_f?
3. What is the difference between RecJ_f and Exonuclease I (NEB# M0293)?
4. Can RecJ_f be heat inactivated?
5. Will RecJF work in rCutSmart buffer?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0264S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0264S_L_v1_0041709
• M0264S_L_v1_0051804
• M0264L_v1_10014919
• M0264S_v1_10014921
• M0264S_v1_10022546
• M0264L_v1_10035557
• M0264S_v1_10043570
• M0264L_v1_10048158
• M0264S_v1_10048155
• M0264L_v1_10062303
• M0264L_v1_10069210
• M0264S_v1_10070012
• M0264L_v2_10092597
• M0264S_v2_10091327
• M0264L_v2_10104349
• M0264S_v2_10113736
• M0264L_v2_10113737
• M0264S_v2_10130289
• M0264S_v2_10137397
• M0264L_v2_10130291
• M0264L_v2_10144524
• M0264S_v2_10150791
• M0264L_v2_10150792
• M0264L_v2_10178452
• M0264S_v2_10178533

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• RecJ_f
• NEBuffer™ 2

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.