Unit Definition

One unit is defined as the amount of enzyme required to produce 0.05 nmol TCA soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 2 with 1.5 µg sonicated single-stranded [³H]-labeled E. coli DNA.

Reaction Conditions

1X NEBuffer™ 2
Incubate at 37°C

1X NEBuffer™ 2
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl₂
1 mM DTT
(pH 7.9 @ 25°C)

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 min

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• NEBuffer 2

1. Lovett, S.T., Kolodner, R.D. (1989). Proc. Natl. Acad. Sci. USA. 86, 2627-2631.
2. Lovett, S. and Whitaker, R. Unpublished observation
3. Whitaker, R. Unpublished observation

1. What is the activity of RecJ_f in the NEBuffers?
2. Can DNA be blunted using RecJ_f?
3. What is the difference between RecJ_f and Exonuclease I (NEB# M0293)?
4. Can RecJ_f be heat inactivated?
5. Will RecJF work in CutSmart buffer?

• Properties of Exonucleases and Endonucleases

• NEBioCalculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• RecJ_f
• NEBuffer™ 2

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.