ShortCut® RNase III
ShortCut RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA).
- Isolated from a recombinant source
- Useful for gene silencing and target validation
- Supplied with 10X Reaction Buffer
ShortCut® RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells (1–3). 1.5 units (1 µl) of ShortCut RNase III is sufficient to convert 1 µg of dsRNA into siRNA suitable for RNA interference in mammalian cells.
Product SourceAn E. coli strain containing a genetic fusion of the E. coli RNase III gene (rnc) and the gene coding for maltose binding protein (MBP).
The following reagents are supplied with this product:
Store at (°C) Concentration Shortcut Reaction Buffer 10 X 10X EDTA 500 mM MnCl2 10 X Glycogen RNase-Free 10 mg/ml
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