9°N™ DNA Ligase

The M0238L (large size only) will be discontinued on 1/1/18.

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Ordering Information

  • 40,000 units/ml
    2,500 units
    $76.00
  • 40,000 units/ml
    12,500 units
    $304.00
  • Product Information
    9° N™ DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands that are hybridized and accurately paired, with no gap, to a complementary DNA strand.  9° N DNA Ligase uses ATP as a cofactor and it is active at elevated temperatures (45° C – 70° C).

    Highlights

    • Can be used to ligate nicks in DNA while incubating at high temperatures
    • Extremely thermostable and can withstand PCR conditions
    • Will not ligate short 4 base overlaps (typical of restriction enzyme digests), while it efficiently ligates 12 base pair overlaps
    • Isolated from a recombinant source

    Product Source

    Purified from an E. coli strain containing the cloned ligase gene from the extremely thermophilic marine archaea Thermococcus sp.(strain 9°N). The archaea was isolated from a submarine thermal vent, at a depth of 2,500 meters, 9° north of the equator at the East Pacific Rise (1).

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    9°N DNA Ligase Reaction Buffer -20 10 X
    Product Categories:
    DNA Ligase Products
    Applications:
    Cloning Ligation,
    DNA Ligation
    • Advantages and Features
    • Properties and Usage
    • Notes
    • References
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