9°N™ DNA Ligase
- Can be used to ligate nicks in DNA while incubating at high temperatures
- Extremely thermostable and can withstand PCR conditions
- Will not ligate short 4 base overlaps (typical of restriction enzyme digests), but efficiently ligates 12 base pair overlaps
- Isolated from a recombinant source
9° N™ DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands that are hybridized and accurately paired, with no gap, to a complementary DNA strand. 9° N DNA Ligase uses ATP as a cofactor and it is active at elevated temperatures (45° C – 70° C).
Product SourcePurified from an E. coli strain containing the cloned ligase gene from the extremely thermophilic marine archaea Thermococcus sp.(strain 9°N). The archaea was isolated from a submarine thermal vent, at a depth of 2,500 meters, 9° north of the equator at the East Pacific Rise (1).
The following reagents are supplied with this product:
Store at (°C) Concentration 9°N DNA Ligase Reaction Buffer -20 10 X
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