Protocol for 9°N DNA Ligase (M0238)

1. Prepare reaction set-up as follows:
DNA up to 1 µg
9°N DNA Ligase Reaction Buffer (10X)
5 µl
9°N DNA Ligase 2 µl (80 units)
H2O up to 50 µl

2. Incubate at 45°C for 15 minutes.
  1. NEBioCalculator Ligations Thumbnail

    NEBioCalculator® - Using the Ligation module

    This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction.