T4 RNA Ligase 1 (ssRNA Ligase)
Avoiding RNase Contamination
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T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates (1).
Product SourceAn E. Coli strain that carries the T4 RNA Ligase I gene
The following reagents are supplied with this product:
Store at (°C) Concentration T4 RNA Ligase Reaction Buffer -20 Adenosine-5'-Triphosphate (ATP) -20 10 mM PEG 8000 -20 50 %
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