T4 RNA Ligase 1 (ssRNA Ligase)
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid to a 3' hydroxyl-terminated nucleic acid. Substrates include single-stranded RNA and DNA, as well as dinucleoside pyrophosphates.
- Ligation of ss-RNA and DNA
- Labeling of 3'-termini of RNA with 5'-[32P] pCp
- Synthesis of single-stranded oligodeoxyribo-nucleotides
- Recombinant enzyme supplied with 10X Reaction Buffer, ATP and PEG
- Tested for the absence of endonucleases, exonocleases and RNases
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
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T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates (1).
Product SourceAn E. Coli strain that carries the T4 RNA Ligase I gene
The following reagents are supplied with this product:
Store at (°C) Concentration T4 RNA Ligase Reaction Buffer -20 10 X Adenosine-5'-Triphosphate (ATP) -20 10 mM PEG 8000 -20 50 %
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