The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA.
The NEBNext Ultra II Ligation Module
is Designed for use with the Following:
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
- NEBNext Ultra II Q5® Master Mix (NEB #M0544)
- NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535)
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
|Store at (°C)||Concentration
|NEBNext Ultra II Ligation Master Mix||-20||
|NEBNext Ligation Enhancer||-20||