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NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®

Don’t be biased! The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.

  • Includes NEBNext FS (Fragmentation System) enzymatic fragmentation reagents
  • Perform fragmentation, end repair and dA-tailing with a single enzyme mix 
  • Save time with an optimized PCR-free workflow, reduced hands-on time and automation compatibility
  • Generate high quality libraries without an amplification step using inputs from 50 ng to 500 ng intact DNA
  • Benefit from improved library uniformity
  • Use with the NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) or other Illumina-compatible index adaptors with 3′ single T overhang

For convenience, a version of this kit is available with SPRIselect beads.

Note: For sheared DNA, we recommend the NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina.

Catalog # Concentration Size
E7430S Not Applicable 24 reactions
E7430L Not Applicable 96 reactions
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