PURExpress® Δ RF123 Kit
PURExpress® Δ RF123 kit is a variation of the PURExpress In vitro Protein Synthesis Kit where the release factors 1, 2 and 3 are omitted from the translation mix.
- RF1, RF2 and RF3 are supplied separately to allow users to control protein synthesis with/without release factors of their choice
- Essentially RF1 and RF3 free, RF2 is greatly reduced to minimal levels
- Allows for robust recovery of activity-encoding nucleic acid template in library screening applications
- Applications include non-natural amino acid incorporation, ribosome display and mRNA display
A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
The following reagents are supplied with this product:
Store at (°C) Concentration PURExpress Solution A -80 Control (DHFR) template (10 μl) -80 Solution B (75 μl) -80 3.3 X RF1 (10 μl) -80 RF2 (10 μl) -80 RF3 (10 μl) -80
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