ProtoScript® II First Strand cDNA Synthesis Kit
The ProtoScript® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA.
- Recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability (active up to 48°C)
- Generates cDNA up to 10 kb
- Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free water
Avoiding RNase Contamination
ProtoScript II First Strand cDNA Synthesis Kit features two optimized mixes, ProtoScript II Enzyme Mix and ProtoScript II Reaction Mix. The enzyme mix combines ProtoScript II Reverse Transcriptase and Murine RNase Inhibitor, while the reaction mix contains dNTPs and an optimized buffer. ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity and higher yield of cDNA.
The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.
The following reagents are supplied with this product:
Store at (°C) Concentration Nuclease-free Water -20 ProtoScript® II Reaction Mix -20 2 X ProtoScript® II Enzyme Mix -20 10 X Oligo d(T)23 VN -20 50 µM Random Primer Mix -20 60 µM
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