Home FAQs How do I choose between Induro Reverse Transcriptase and the different LunaScript and ProtoScript products?

FAQ: How do I choose between Induro Reverse Transcriptase and the different LunaScript and ProtoScript products?

Induro Reverse Transcriptase is a group II intron-encoded RT that is useful for cDNA synthesis from long transcripts, RNAs with strong secondary structures, and RNA samples with inhibitors. In contrast, the LunaScript reagents are master mixes. The LunaScript RT Master Mix Kit (Primer-free) (NEB #E3025) is suitable for general cDNA synthesis. LunaScript RT SuperMix products (NEB #E3010/M3010) are optimized for two step RT-qPCR workflows where only short cDNA products are required. LunaScript RT SuperMix is also featured in the ARTIC SARS-CoV-2 sequencing workflow. This table outlines the applications and features of each.

 Intron-encoded RT  Retroviral RT Products
Induro™ Reverse Transcriptase
(NEB #M0681)

LunaScript® RT SuperMix Kit (NEB #E3010)

LunaScript RT SuperMix (NEB #M3010) 

 LunaScript RT Master Mix Kit (NEB #E3025) ProtoScript® II First Strand cDNA Synthesis Kit (NEB #E6560) 

 Applications

  • Long cDNA synthesis
  • Impure RNA samples
  • RNA-seq applications (e.g., direct RNA sequencing, long-read cDNA sequencing, tRNA seq

 Applications

  • 2-step RT-qPCR detection]
  • RNA-seq workflow

Applications

  • RNA-seq workflow
  • 2-step RT-qPCR detection
  • General cDNA synthesis
  • 2-step RT-PCR detection

 Applications

  • General cDNA synthesis
  • RNA-seq workflow
  • 2-step RT-PCR detection
✓ Short protocol
✓ Flexible choice of primers, modified dNTPs
✓ Higher reaction temperature		 ✓ Supermix
✓ Short protocol
✓ Tracking dye
✓ Higher reaction temperature		 ✓ Flexible choice of primers
✓ Short protocol
✓ Tracking dye
✓ Higher reaction temperature		 ✓ Flexible choice of primers
✓ Kit format includes all necessary components