NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module
Kits are also available that include reagents for every step in the non-directional Ultra II RNA library construction workflow (NEBNext® Ultra II RNA Library Prep Kit for Illumina, NEB #E7770 and NEBNext Ultra II RNA Library Prep with Sample Purification Beads, NEB #E7775).
The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow.
The NEBNExt Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:
- NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) or NEBNext RNA First Strand Synthesis Module (NEB #E7525)
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7442)
- NEBNext Ultra II Ligation Module (NEB #E7595) or NEBNext Ultra Ligation Module (NEB #E7445)
- NEBNext Ultra™ II Q5® Master Mix (NEB #M0544) or NEBNext High Fidelity 2X PCR Master Mix (NEB #M0541)
- NEBNext® Oligos for Illumina® (#E7535, NEB #E7350, NEB #E7335, #E7500, #E7710, #E7730, #E7600, #E6609)
For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact [email protected] for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext® Second Strand Synthesis Enzyme Mix -20 NEBNext® Second Strand Synthesis Reaction Buffer -20
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- Reverse Transcriptases & RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases Products,
- RNA Library Prep Products
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