OneTaq® RT-PCR Kit
Easy and efficient two-step RT-PCR
- Optimized reaction mixes for cDNA synthesis and PCR amplification
- Hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- OneTaq® DNA Polymerase delivers robust amplification across a wide range of templates
Avoiding RNase Contamination
OneTaq® RT-PCR Kit combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions.
The first strand cDNA synthesis is achieved by using two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix. M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and murine RNase Inhibitor while M-MuLV Reaction Mix contains dNTPs and an optimized buffer. The kit also contains two optimized primers for reverse transcription and nuclease-free water. An anchored oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs.
The amplification step features a OneTaq Hot Start DNA Polymerase in a master mix format. OneTaq Hot Start DNA Polymerase offers higher fidelity than Taq and better amplification. RT-PCR product up to 6 kb can be generated (Figure 1).
The following reagents are supplied with this product:
Store at (°C) Concentration Nuclease-free Water -20 M-MuLV Enzyme Mix -20 10 X M-MuLV Reaction Mix -20 2 X OneTaq® Hot Start 2X Master Mix with Standard Buffer -20 2 X Oligo d(T)23 VN -20 50 μM Random Primer Mix -20 60 μM
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