NEB® Golden Gate Assembly Mix

This product has been discontinued and replaced by NEBridge® Golden Gate Assembly Kit (BsaI-HF®v2) (NEB #E1601)

  • Catalog # E1600 was discontinued on October 18, 2018
  • Seamless cloning – no scar remains following assembly
  • Includes destination plasmid with T7/SP6 promoters
  • Ordered assembly of multiple fragments in a single reaction
  • Can also be used for cloning of single inserts
  • Efficient with regions with high GC content and areas of repeats
  • Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)
  • Free tool available at

Storage Temperature:  -20°C

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