HomeFAQsFor amplicon inserts, why are the calculations suggested as using the overall
pGGA destination plasmid length and the overall amplicon lengths? Shouldn’t
only the part of pGGA functioning as the vector backbone be used?
FAQ: For amplicon inserts, why are the calculations suggested as using the overall
pGGA destination plasmid length and the overall amplicon lengths? Shouldn’t
only the part of pGGA functioning as the vector backbone be used?
It can, but the length of the vector backbone in pGGA differs only by 41 bp
from its full plasmid length; since this 41 bp “fall away” fragment closely
approximates the lengths of the 5´ flanking bases and BsaI sites on each
amplicon insert, the simpler overall plasmid:insert calculation can be used.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.