FAQ: Can the Golden Gate Assembly reactions be scaled down?

Except for very complex, multi-insert assembly reactions, the reactions can be scaled down 2–3 fold if input volumes allow. This can be done by scaling down the reaction volume and components proportionately, or keeping the 20 µl reaction volume but using 2–3 fold less of each DNA component. In this latter case plate a larger amount of outgrowth to compensate.