HomeFAQsCan the Golden Gate Assembly reactions be scaled down?
FAQ: Can the Golden Gate Assembly reactions be scaled down?
Except for very complex, multi-insert assembly reactions, the reactions can be
scaled down 2–3 fold if input volumes allow. This can be done by scaling down
the reaction volume and components proportionately, or keeping the 20 µl
reaction volume but using 2–3 fold less of each DNA component. In this latter
case plate a larger amount of outgrowth to compensate.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
