S-adenosylmethionine (SAM)

Description

S-adenosyl-methionine (SAM) is included with all of the DNA methylases available from New England Biolabs, Inc. As well as certain restriction enzymes for which it is required. The SAM is prepared in a solution of 0.005 M H2SO4 and 10% ETOH and filtered.

Properties and Usage

Storage Temperature

-20°C

Notes

  1. For use of methylation reaction the SAM should be diluted 1:400 to a final concentration of 80μM.

FAQs

  1. What is the source of your S-adenosyl-methionine (SAM)?
  2. What is the buffer composition of your S-adenosyl-methionine (SAM)?
  3. Will the buffer from the SAM inhibit my reaction?
  4. What quality controls are performed on your SAM?
  5. Is SAM supplied with enzymes that require it in the reaction buffer?
  6. How stable is SAM in a reaction?
  7. Should I replenish the SAM in my reaction?
  8. How long can I store SAM?
  9. Why is my Restriction Enzyme not cutting DNA?
  10. Why do I see additional DNA bands on my gel after a restriction digest?
  11. Why do I see a DNA smear on an agarose gel after a restriction digest?
  12. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Assurance Statement

  • Each lot of SAM is tested in an SssI (CpG) Methylase (NEB #M0226) reaction with λ DNA. The DNA is then challenged with BstUI to confirm methylation.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Health Warning

  • DANGER
  • Causes skin and/or serious eye irritation.
  • Flammable liquid and vapor.
  • Harmful to aquatic life.