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  • Quick-Load® 100 bp DNA Ladder

    Description

    Quick-Load® 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

    The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference bands.

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    100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane.

    Properties and Usage

    Bases

    FragmentMassbp
    1451,517
    2351,200
    3951,000
    427900
    524800
    621700
    718600
    897500/517
    938400
    1029300
    1125200
    1248100

    Effective Size Range

    100bp to 1,517bp

    Storage Temperature

    4°C

    Storage Conditions

    3.3 mM Tris-HCl
    11 mM EDTA
    0.015% bromophenol blue
    0.017% SDS
    2.5% Ficoll®-400
    pH 8.0 @ 25°C

    Notes

    1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
    2. We recommend loading 10 μl (0.5 μg) of Quick-Load 100 bp DNA Ladder per gel lane.
    3. In general, it is recommended to load 1µl per mm of gel lane.
    4. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    5. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
    6. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.) Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use SYBR® with the DNA Ladders from NEB?
    5. Can I use Midori Green with the DNA Ladders from NEB?

    Selection Tools

    Mix well before use, especially after thawing.