The Wellcome Trust Sanger Institute has recently re-evaluated its RNA-seq library preparation methods and has chosen to implement the NEBNext Ultra II Directional RNA Library Prep Kit. Our previous RNA-seq library preparation required a minimum input of 500 ng total RNA, but the high efficiency of the NEBNext Ultra II kit has allowed us to reduce this to either 100 ng for a standard pipeline, or down to just 5 ng for a low input option, enabling RNA-seq to be carried out from just 5,000-10,000 cells.
Available kits:
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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® |
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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads |
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NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® |
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NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads |
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NEBNext Selector Find the right kits for your DNA or RNA sample preparation with our interactive online tool |