Sample Preparation for Next Generation Sequencing
As sequencing technologies improve and applications expand, the need for compatibility with ever-decreasing input amounts and sub-optimal sample quality grows. Scientists must balance reliability and performance with faster turnaround, higher throughput and automation compatibility.
For over 12 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. The NEBNext portfolio includes solutions for DNA and RNA sample prep from a wide range of sample types. Use of NEBNext reagents has been cited in >12,000 peer-reviewed publications.
Most recently, in the COVID-19 pandemic, NEBNext® reagents are being employed in applications including targeted SARS-CoV-2 sequencing, host genomic sequencing and metagenomic sequencing. These include new NEBNext kits for the ARTIC SARS-CoV-2 workflow, compatible with Illumina® and Oxford Nanopore Technologies® sequencing.
Videos
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COVID-19 Researcher Spotlight: Interview with Chris Mason
In this video Chris Mason discusses COVID-19 research and collaboration efforts including global metagenomics studies and SARS-CoV-2 detection via LAMP.
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Highly multiplexed capture of genomic markers for high throughput NGS based genotyping
In this ESHG 2020 workshop, hear about methylation analysis of cfDNA samples, sample identification and tracking using a high-throughput SNP genotyping method and the importance of sample prep in large-scale sequencing pipelines.
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Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
This webinar from NEB®, EMBL and Beckman Coulter® Life Sciences discusses the development and verification of the automated NEBNext Direct® Genotyping Solution on the Beckman Coulter i7 workstation to achieve highly-multiplexed sample processing and meet the growing demands of plant and animal genotyping applications.
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NEBNEXT® Enzymatic Methyl-seq Workflow
A workflow animation for an enzyme-based method for detection of 5mC and 5hmC, an alternative to bisulfite sequencing.
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NEBNEXT® Ultra II Directional RNA Workflow
Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.
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Size Selection and Cleanup with NEBNEXT® Ultra II and SPRI beads
This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA
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Tips for Optimizing DNA Inputs in NGS Library Construction
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
Select your application
- DNA Library Prep – Fast, streamlined workflows and novel reagents for a broad range of input amounts
- FFPE DNA – Repair DNA damage caused by FFPE treatment, and perform high-efficiency library prep
- ChIP-Seq Library Prep – High-efficiency, streamlined workflows for low input amounts of DNA
- RNA Library Prep – Novel reagents and streamlined workflows for directional and non-directional RNA, depletion of abundant RNAs (rRNA and mRNA), mRNA enrichment, and single cells
- Target Enrichment – High-quality library generation in a single day with the unique hybridization-based NEBNext Direct® technology
- Microbiome DNA Enrichment – Separation of microbiome DNA from contaminating host DNA from low input amounts
- Library Quantitation – Accurate and reproducible quantitation of Illumina libraries, with a user-friendly workflow
Scroll through to see what we’ve been up to over the past 10 years!
Featured NEBNext® products
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Featured Tools and Resources
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NEBNext® Custom RNA Depletion Design Tool |
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