Sample Preparation for Next Generation Sequencing

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As sequencing technologies improve and applications expand, the need for compatibility with ever-decreasing input amounts and sub-optimal sample quality grows. Scientists must balance reliability and performance with faster turnaround, higher throughput and automation compatibility.

For over 10 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. The NEBNext portfolio includes solutions for DNA and RNA sample prep from a wide range of sample types.

Most recently, in the COVID-19 pandemic, NEBNext reagents are being employed in applications including targeted SARS-CoV-2 sequencing, host genomic sequencing and metagenomic sequencing.

COVID_protocol_Icon   More information on these and other protocols

Benefits of NEBNext®

  • A broad selection of kits for sample preparation of DNA, ChIP DNA, and RNA, including single cell and small RNA
  • Optimized formulations for reduced workflow times and ever-decreasing input amounts
  • Extensive quality controls and functional validation for all kits
  • Multiple workflow formats for maximum convenience and flexibility
  • Best-in-class technical support from NEB scientists and staff
  • A proven track record, with mention in over 9,000 publications to-date

  1. Fully-Automated-Targeted-SNP-Genotyping-for-Plant-and-Animal-Applications

    Fully Automated Targeted SNP Genotyping for Plant and Animal Applications

    This webinar from NEB®, EMBL and Beckman Coulter® Life Sciences discusses the development and verification of the automated NEBNext Direct® Genotyping Solution on the Beckman Coulter i7 workstation to achieve highly-multiplexed sample processing and meet the growing demands of plant and animal genotyping applications.

  2. EMseqWorkflow_Animation_Thumb_720

    NEBNext® Enzymatic Methyl-seq Workflow

    A workflow animation for an enzyme-based method for detection of 5mC and 5hmC, an alternative to bisulfite sequencing.

  3. NEBNextUltra2DirectionalRNA_720

    NEBNext Ultra II Directional RNA Workflow

    Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.

  4. Size Selection and Cleanup with NEBNext Ultra

    Size Selection and Cleanup with NEBNext Ultra II and SPRI beads

    This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA

  5. NEBNextVideo_OptimizingDNAInput_thumb

    Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.

Select your application

  • DNA Library Prep – Fast, streamlined workflows and novel reagents for a broad range of input amounts
  • FFPE DNA – Repair DNA damage caused by FFPE treatment, and perform high-efficiency library prep
  • ChIP-Seq Library Prep – High-efficiency, streamlined workflows for low input amounts of DNA
  • RNA Library Prep – Novel reagents and streamlined workflows for directional and non-directional RNA, depletion of abundant RNAs (rRNA and mRNA), mRNA enrichment, and single cells
  • Target Enrichment – High-quality library generation in a single day with the unique hybridization-based NEBNext Direct® technology
  • Microbiome DNA Enrichment – Separation of microbiome DNA from contaminating host DNA from low input amounts
  • Library Quantitation – Accurate and reproducible quantitation of Illumina libraries, with a user-friendly workflow

Scroll through to see what we’ve been up to over the past 10 years!

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

Illumina® is a registered trademark of Illumina, Inc. Ion Torrent™ is a trademark of Life Technologies, Inc.

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Did you know that many of these products can be purchased in larger volumes? Contact us to learn more.