Sample Preparation for Next Generation Sequencing


Library preparation is a critical part of the next generation sequencing workflow; successful sequencing requires the generation of high quality libraries of sufficient yield and quality from ever-decreasing input quantities as well as from samples of lower quality. At the same time, there is a need for faster, automatable protocols that perform reliably without compromising the quality of the libraries produced.

To meet these growing challenges, the NEBNext suite of products continues to evolve to support next generation sequencing with sample preparation tools that streamline workflows, minimize inputs, and improve library yields and quality.

Benefits of NEBNext®

  • A broad selection of kits for sample preparation of DNA, ChIP DNA, and RNA, including single cell and small RNA
  • Optimized formulations for reduced workflow times and ever-decreasing input amounts
  • Extensive quality controls and functional validation for all kits
  • Multiple workflow formats for maximum convenience and flexibility
  • Best-in-class technical support from NEB scientists and staff
  • A proven track record, with mention in over 1,000 publications to-date
  1. NEBNextUltra2DirectionalRNA_720

    NEBNext Ultra II Directional RNA Workflow

    Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.

  2. Size Selection and Cleanup with NEBNext Ultra

    Size Selection and Cleanup with NEBNext Ultra II and SPRI beads

    This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA

  3. NEBNextVideo_OptimizingDNAInput_thumb

    Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

Select your application

  • DNA Library Prep – Fast, streamlined workflows and novel reagents for a broad range of input amounts
  • FFPE DNA – Repair DNA damage caused by FFPE treatment, and perform high-efficiency library prep
  • ChIP-Seq Library Prep – High-efficiency, streamlined workflows for low input amounts of DNA
  • RNA Library Prep – Novel reagents and streamlined workflows for directional and non-directional RNA, rRNA depletion, mRNA enrichment, and single cells
  • Target Enrichment – High-quality library generation in a single day with the unique hybridization-based NEBNext Direct® technology
  • Microbiome DNA Enrichment – Separation of microbiome DNA from contaminating host DNA from low input amounts
  • Library Quantitation – Accurate and reproducible quantitation of Illumina libraries, with a user-friendly workflow



One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

Illumina® is a registered trademark of Illumina, Inc. Ion Torrent™ is a trademark of Life Technologies, Inc.

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Did you know that many of these products can be purchased in larger volumes? Contact us to learn more.