Sample Preparation for Next Generation Sequencing

As sequencing technologies improve and applications expand, the need for compatibility with ever-decreasing input amounts and sub-optimal sample quality grows. Scientists must balance reliability and performance with faster turnaround, higher throughput and automation compatibility.
For over 11 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. The NEBNext portfolio includes solutions for DNA and RNA sample prep from a wide range of sample types. Use of NEBNext reagents has been cited in >11,000 peer-reviewed publications.
Most recently, in the COVID-19 pandemic, NEBNext® reagents are being employed in applications including targeted SARS-CoV-2 sequencing, host genomic sequencing and metagenomic sequencing. These include new NEBNext kits for the ARTIC SARS-CoV-2 workflow, compatible with Illumina® and Oxford Nanopore Technologies® sequencing.
Select your application
- DNA Library Prep – Fast, streamlined workflows and novel reagents for a broad range of input amounts
- FFPE DNA – Repair DNA damage caused by FFPE treatment, and perform high-efficiency library prep
- ChIP-Seq Library Prep – High-efficiency, streamlined workflows for low input amounts of DNA
- RNA Library Prep – Novel reagents and streamlined workflows for directional and non-directional RNA, depletion of abundant RNAs (rRNA and mRNA), mRNA enrichment, and single cells
- Target Enrichment – High-quality library generation in a single day with the unique hybridization-based NEBNext Direct® technology
- Microbiome DNA Enrichment – Separation of microbiome DNA from contaminating host DNA from low input amounts
- Library Quantitation – Accurate and reproducible quantitation of Illumina libraries, with a user-friendly workflow
Scroll through to see what we’ve been up to over the past 10 years!
Featured NEBNext® products
Request a SampleFeatured Tools and Resources
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NEBNext® Custom RNA Depletion Design Tool |
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NEBNext Selector For help with selecting a product, try our interactive online tool |