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Next Generation Sequencing Library Preparation

Successful Next Generation Sequencing (NGS) hinges on several factors, but one of the most impactful of them is the choice of library preparation workflow. As NGS matures into an essential analysis method, libraries of the highest quality are required, in yields sufficient for a wide range of applications and throughputs, from single-cell and cfDNA to PCR-free whole-genome analysis. Automation compatibility is a major consideration, too, as liquid-handling robots are becoming more accessible and commonplace.

NEBNext^® Ultra^TM II workflow for a wide range of sample types

For over 13 years, NEB has addressed these challenges by offering solutions that streamline sample prep workflows, minimize inputs and improve library yield and quality. With over 90 products, the NEBNext portfolio includes sample prep solutions for DNA sequencing and RNA sequencing from a wide range of sample types. The NEBNext Ultra II workflow lies at the heart of the portfolio, and products are available in a convenient kit format, or as separate modules – offering flexible library prep workflows that are easily scaled and automated.

NEBNext reagents are currently available for next generation sequencing sample preparation on Illumina^®, Oxford Nanopore Technologies^® (ONT) and IonTorrent^® sequencing platforms, and have already been applied to emerging sequencing platforms.

What’s next for your NGS Library Prep? We’ve got you covered!

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Next Generation Sequencing Library Preparation includes these subcategories:

Library Preparation for Illumina
Library Preparation for Ion Torrent
Library Preparation for Oxford Nanopore Technologies
Infectious Diseases & ARTIC Sequencing
DNA Fragmentation & RNA Fragmentation
RNA Depletion & mRNA Enrichment
Methylome Analysis
Microbiome DNA Enrichment
NEBNext^® Multiplex Oligos (Adaptors & Primers)
FFPE DNA Repair
NGS Library Quantitation
Automation for NEBNext^® NGS Library Prep
Small RNA Library Preparation

Application Notes for Next Generation Sequencing Library Preparation

Tools & Resources

Feature Articles

Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction

Among the available options for DNA fragmentation, enzymatic fragmentation is demonstrably gentler on the sample, yielding less damage at any scale. The new NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® includes a single step for enzymatic fragmentation, end repair, and dA tailing.

Advances in next generation sequencing: How researchers at NEB are working to improve our understanding of the genome, epigenome and transcriptome.

NEB scientists develop sequencing technologies to analyze genome-wide methylation, transcriptional start sites, full-length transcriptomes, chromatin accessibility, and much more.

Posters

Induro™, a Novel Reverse Transcriptase for Nanopore Direct RNA Sequencing with Significantly Improved RNA 5′ Coverage

Legal Information

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Videos

How Library Preparation Affects Sequencing Accuracy

In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary.